Buses will run normally in both directions during phases one and two of Brighton & Hove City Council's Valley Gardens project, which starts on Monday 3 September and is expected to take up to 24 months.
Brighton & Hove Buses would like to reassure passengers that their buses will continue to run as usual during the multi-million-pound project, which spans the area from St Peter's Church to Edward Street and will create new public open space.
Brighton & Hove Buses' Managing Director Martin Harris said: "We want to reassure passengers that buses will continue to run both ways throughout the Valley Gardens project. We will be working closely with the City Council to ensure that there is minimal disruption to passengers' journeys as building work progresses.
"Valley Gardens is part of the vision for a more people-friendly, cleaner and more communal space. It will open up this part of Brighton and make it more attractive to visitors and residents alike.
"The end result will be extra green space; a simplified road layout with better links to the city centre and better access for pedestrians, cyclists and buses; as well as making it safer and easier to cross the road."
There will be extra Brighton & Hove Buses' supervisory staff on the ground to ensure buses run as smoothly as possible.
When complete, the project will mean that traffic and bus lanes along the A23 from St Peter's Church to the junction of Church Street will be rerouted and traffic flows will change.
Buses and taxis will have a two-lane corridor - one in each direction - on the west side of the gardens, on Marlborough Place and Gloucester Place. Only local traffic turning left into North Laine side streets will be permitted on this side.
General traffic will be rerouted to the east of the gardens along Grand Parade. While there will be two lanes for cars going south, this is reduced to one between Richmond Parade and North Road. There will be one lane for general traffic going north.
Work begins on Monday 3 September and civil engineers Dyer and Butler estimate the work will take up to 24 months.
Proposals for Phase 3 of the works, from the Old Steine to the Palace Pier roundabout are currently being developed.